Utilization of organophosphate:phosphate antiporter for isotope-labeling experiments inE. coli

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Reconciling Estimates of Cell Proliferation from Stable Isotope Labeling Experiments

Stable isotope labeling is the state of the art technique for in vivo quantification of lymphocyte kinetics in humans. It has been central to a number of seminal studies, particularly in the context of HIV-1 and leukemia. However, there is a significant discrepancy between lymphocyte proliferation rates estimated in different studies. Notably, deuterated (2)H2-glucose (D2-glucose) labeling stud...

متن کامل

IsoCor: correcting MS data in isotope labeling experiments

UNLABELLED Mass spectrometry (MS) is widely used for isotopic labeling studies of metabolism and other biological processes. Quantitative applications-e.g. metabolic flux analysis-require tools to correct the raw MS data for the contribution of all naturally abundant isotopes. IsoCor is a software that allows such correction to be applied to any chemical species. Hence it can be used to exploit...

متن کامل

Labeling of Human Serum Albumin with Stable Isotope of Bromine; an in Vitro Study

Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...

متن کامل

Isotope Labeling Methods for Protein Dynamics Studies

Protein structure determination by solution NMR spectroscopy has long relied on the uniform stable isotopic enrichment with 13C and 15N to alleviate resonance overlap and to allow multiple distance and angular restraints, at as many atomic sites as possible, to facilitate computing the optimal three-dimensional structural model.(1) Recently, the optimization of these labeling techniques has inc...

متن کامل

An efficient and cost-effective isotope labeling protocol for proteins expressed in Escherichia coli.

A cost-effective protocol for uniform 15N and/or 13C isotope labeling of bacterially expressed proteins is presented. Unlike most standard protocols, cells are initially grown in a medium containing nutrients at natural abundance and isotopically labeled nutrients are only supplied at the later stages of growth and during protein expression. This permits the accumulation of a large cell mass wi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 2014

ISSN: 0378-1097

DOI: 10.1111/1574-6968.12612